In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 12
... Coverslips Round coverslips are used only once . The coverslips are placed in Chen racks for their initial overnight soaking in 10 % HCl . After rinsing in deionized water , they are transferred in the racks to concentrated nitric acid ...
... Coverslips Round coverslips are used only once . The coverslips are placed in Chen racks for their initial overnight soaking in 10 % HCl . After rinsing in deionized water , they are transferred in the racks to concentrated nitric acid ...
Page 19
... coverslip forceps and two scalpels with No. 11 blades . Also on the lower tier are two 20 × 150 mm petri dishes lined with black filter paper , a 15 × 100 mm petri dish with processed and ster- ilized square coverslips , an empty 15 ...
... coverslip forceps and two scalpels with No. 11 blades . Also on the lower tier are two 20 × 150 mm petri dishes lined with black filter paper , a 15 × 100 mm petri dish with processed and ster- ilized square coverslips , an empty 15 ...
Page 64
... Coverslip Cleaning Using a pair of forceps , 60 coverslips are submerged individually in dilute deter- gent ( 10 ml of a 5 % solution of 7X , Flow Laboratories , McLean , VA ) in a small ( 100 ml ) filtration Erlenmeyer flask . The ...
... Coverslip Cleaning Using a pair of forceps , 60 coverslips are submerged individually in dilute deter- gent ( 10 ml of a 5 % solution of 7X , Flow Laboratories , McLean , VA ) in a small ( 100 ml ) filtration Erlenmeyer flask . The ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml