In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 218
... factors , pH buffers , protease inhibitors , and binding proteins which may modulate the action of vitamins , lipids , and metals , or detoxify the medium ( 17 ) . It is possible that undefined components of serum may mask the effect of ...
... factors , pH buffers , protease inhibitors , and binding proteins which may modulate the action of vitamins , lipids , and metals , or detoxify the medium ( 17 ) . It is possible that undefined components of serum may mask the effect of ...
Page 381
... factor called hepatocyte growth factor ( 22 ) are potent mitogens for rat kidney proximal tubule epithelial cells . It is immediately obvious that the nomenclature for growth factors is often not a reflection of their spectrum of action ...
... factor called hepatocyte growth factor ( 22 ) are potent mitogens for rat kidney proximal tubule epithelial cells . It is immediately obvious that the nomenclature for growth factors is often not a reflection of their spectrum of action ...
Page 560
... factors , and supple- ments for , 376 preparation of extracellular matrix protein - coated dishes for , 378 procedures for , 376-380 purity of , determination of , 379–380 Rat renal proximal tubule epithelial cells , 374 changes in ...
... factors , and supple- ments for , 376 preparation of extracellular matrix protein - coated dishes for , 378 procedures for , 376-380 purity of , determination of , 379–380 Rat renal proximal tubule epithelial cells , 374 changes in ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml