In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 260
... hepatocytes from other laboratory species including mouse , hamster , and rabbit ( 13,14 ) . A second area where hepatocytes have proved useful is in studies of chemical biotransformation , particularly species comparisons . For example ...
... hepatocytes from other laboratory species including mouse , hamster , and rabbit ( 13,14 ) . A second area where hepatocytes have proved useful is in studies of chemical biotransformation , particularly species comparisons . For example ...
Page 273
... hepatocytes can be obtained from whole liv- ers , 1 to 15 × 108 from a portion of liver and 2 to 8 × 107 from small wedge biopsies . In most cases cell viability ranges between 70 and 90 % . Hepatocyte Culture Freshly isolated human ...
... hepatocytes can be obtained from whole liv- ers , 1 to 15 × 108 from a portion of liver and 2 to 8 × 107 from small wedge biopsies . In most cases cell viability ranges between 70 and 90 % . Hepatocyte Culture Freshly isolated human ...
Page 286
... hepatocytes should have a viability of no less than 80-85 % if they are to be used successfully . Preparing Hepatocytes for Culture As already stated above , the hepatocytes at this point are very fragile and there- fore should be ...
... hepatocytes should have a viability of no less than 80-85 % if they are to be used successfully . Preparing Hepatocytes for Culture As already stated above , the hepatocytes at this point are very fragile and there- fore should be ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml