In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 414
... incubated at 37 ° C in the incubator until they are released from the dish . Overtrypsinization will result in damage to healthy cells ; therefore , this process must be monitored closely by microscopic exami- nation . Immediately after ...
... incubated at 37 ° C in the incubator until they are released from the dish . Overtrypsinization will result in damage to healthy cells ; therefore , this process must be monitored closely by microscopic exami- nation . Immediately after ...
Page 462
... incubated dermal side down on top of 10 ml of 0.5 % trypsin in a sterile tissue culture plate at 37 ° C and 5 % CO2 for 60 min . After incubation , the epidermis is scraped from the dermis using watchmak- ers ' forceps in HBSS with 10 ...
... incubated dermal side down on top of 10 ml of 0.5 % trypsin in a sterile tissue culture plate at 37 ° C and 5 % CO2 for 60 min . After incubation , the epidermis is scraped from the dermis using watchmak- ers ' forceps in HBSS with 10 ...
Page 487
... incubated overnight at 4 ° C . The primary antibody is poured off , and nonspecific sites are blocked by a 1.5 - hr incubation with buffer that contains 5 % nonfat milk in 0.05 % Tween / PBS ( i.e. , blocking buffer ) . The plate is ...
... incubated overnight at 4 ° C . The primary antibody is poured off , and nonspecific sites are blocked by a 1.5 - hr incubation with buffer that contains 5 % nonfat milk in 0.05 % Tween / PBS ( i.e. , blocking buffer ) . The plate is ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml