In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 159
... layers . The innermost layer , or tunica intima , consists of a single layer of endothelial cells which rests on a loose layer of connective tissue formed by a thin basal lamina and a subendothelial layer . This layer acts as a ...
... layers . The innermost layer , or tunica intima , consists of a single layer of endothelial cells which rests on a loose layer of connective tissue formed by a thin basal lamina and a subendothelial layer . This layer acts as a ...
Page 204
... layer of mucosa covered by a whitish , opaque , upper layer of muscle , fat , and serosa . With a pair of forceps in each hand , one begins at the left - hand corner by gently lifting the muscle layer upward and the mucosal layer ...
... layer of mucosa covered by a whitish , opaque , upper layer of muscle , fat , and serosa . With a pair of forceps in each hand , one begins at the left - hand corner by gently lifting the muscle layer upward and the mucosal layer ...
Page 496
... layer allowed to develop over a 7- to 10 - day period . On the initial day of agar coculture , all medium is removed from the adherent layer , and fresh bone marrow cells ( 2 × 105 cells / ml ) in 0.5 ml of culture medium are mixed with ...
... layer allowed to develop over a 7- to 10 - day period . On the initial day of agar coculture , all medium is removed from the adherent layer , and fresh bone marrow cells ( 2 × 105 cells / ml ) in 0.5 ml of culture medium are mixed with ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml