In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
From inside the book
Results 1-3 of 89
Page 237
... liver . Care should be taken to avoid uncontrolled distension of the organ , but the washout efficiency may be aided by intermittently increasing and decreasing the liver pressure by placing a fingertip at the caval efflux site , or by ...
... liver . Care should be taken to avoid uncontrolled distension of the organ , but the washout efficiency may be aided by intermittently increasing and decreasing the liver pressure by placing a fingertip at the caval efflux site , or by ...
Page 314
... liver is washed out with a Ca2 * . free solution followed by perfusion with a collagenase solution . Step 1 : Preliminary Setup for Hepatocyte Isolation . Both solution I and solu ... liver in solution II 314 VIC . Liver : Other Cell Systems.
... liver is washed out with a Ca2 * . free solution followed by perfusion with a collagenase solution . Step 1 : Preliminary Setup for Hepatocyte Isolation . Both solution I and solu ... liver in solution II 314 VIC . Liver : Other Cell Systems.
Page 321
... liver . 4. Using sewing needles with silk , position two sutures under the esophagus between the liver and the stomach , leaving space between the sutures . Tie off sutures and cut through esophagus in between ties . Avoid cutting ...
... liver . 4. Using sewing needles with silk , position two sutures under the esophagus between the liver and the stomach , leaving space between the sutures . Tie off sutures and cut through esophagus in between ties . Avoid cutting ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml