In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 63
... medium : An aliquot ( 50 ml ) of L - 15 supplement mix is thawed , dissolved in 500 ml of Leibowitz L - 15 medium ( GIBCO , 320-1415AJ ) , and stored at 4 ° C . Complete L - 15 growth medium : The 50 - ml tube containing the aliquot ( 5 ...
... medium : An aliquot ( 50 ml ) of L - 15 supplement mix is thawed , dissolved in 500 ml of Leibowitz L - 15 medium ( GIBCO , 320-1415AJ ) , and stored at 4 ° C . Complete L - 15 growth medium : The 50 - ml tube containing the aliquot ( 5 ...
Page 494
... medium . The bone shaft is cut at each end and the medium delivered with a 21 - gauge needle attached to a 5 - ml syringe . Bone marrow plugs are collected in a 60 - mm petri dish ( Falcon 1007 ) , and large clumps are dispersed by ...
... medium . The bone shaft is cut at each end and the medium delivered with a 21 - gauge needle attached to a 5 - ml syringe . Bone marrow plugs are collected in a 60 - mm petri dish ( Falcon 1007 ) , and large clumps are dispersed by ...
Page 497
... medium with glutamine 100.0 ml Procedure LTBMC are established according to a modification of the procedure reported by Whitlock and co - workers ( 14 ) and Whitlock and Witte ( 13 ) . Bone marrow cells are suspended in culture medium ...
... medium with glutamine 100.0 ml Procedure LTBMC are established according to a modification of the procedure reported by Whitlock and co - workers ( 14 ) and Whitlock and Witte ( 13 ) . Bone marrow cells are suspended in culture medium ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml