In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 105
... membrane . The basal membrane of cul- tured RPE makes spot attachments with the plastic surface of the culture plates rather than a continuous contact , basal lamina formation is absent or rudimen- tary , the apical processes are less ...
... membrane . The basal membrane of cul- tured RPE makes spot attachments with the plastic surface of the culture plates rather than a continuous contact , basal lamina formation is absent or rudimen- tary , the apical processes are less ...
Page 406
... membrane . Other cell types in the kidney lack this transport system and thus cannot utilize extra- cellular ... membrane ) or nys- tatin ( which uncouples the Na + , K + -ATPase on the plasma membrane , thereby increasing energy demand ) ...
... membrane . Other cell types in the kidney lack this transport system and thus cannot utilize extra- cellular ... membrane ) or nys- tatin ( which uncouples the Na + , K + -ATPase on the plasma membrane , thereby increasing energy demand ) ...
Page 424
... membrane is completed . To accom- plish these steps , a cut is first made by pinching across the lower third of the ... membrane sur- rounding the VYS ( Fig . 2B ) . These two components are removed simulta- neously by grasping ...
... membrane is completed . To accom- plish these steps , a cut is first made by pinching across the lower third of the ... membrane sur- rounding the VYS ( Fig . 2B ) . These two components are removed simulta- neously by grasping ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml