In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page xvii
... Methods in Toxicology is a new series created to fill this need . These volumes are patterned after the highly regarded Methods in Enzymology series and are intended to provide comprehensive descriptions of state - of - the - art methods ...
... Methods in Toxicology is a new series created to fill this need . These volumes are patterned after the highly regarded Methods in Enzymology series and are intended to provide comprehensive descriptions of state - of - the - art methods ...
Page 110
... methods have been developed for the isolation and culture of alveolar epithelial cells that may permit such examination if appropriate recognition of limitations is consid- ered . The material in this chapter gives a brief overview of ...
... methods have been developed for the isolation and culture of alveolar epithelial cells that may permit such examination if appropriate recognition of limitations is consid- ered . The material in this chapter gives a brief overview of ...
Page 262
... method , two laboratories simultaneously developed perfusion methods for the isolation of human hepato- cytes from wedge - shaped biopsy pieces of liver ( 10 , 11 ) , a technique which has resulted in large yields of highly viable ...
... method , two laboratories simultaneously developed perfusion methods for the isolation of human hepato- cytes from wedge - shaped biopsy pieces of liver ( 10 , 11 ) , a technique which has resulted in large yields of highly viable ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml