In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 90
... Microscopy Morphological actions of neurotoxic agents can be studied in PC - 12 cells by electron microscopy , which allows the changes in subcellular organelles to be easily evaluated ( 12 ) . Organelles to be studied include ...
... Microscopy Morphological actions of neurotoxic agents can be studied in PC - 12 cells by electron microscopy , which allows the changes in subcellular organelles to be easily evaluated ( 12 ) . Organelles to be studied include ...
Page 206
... microscopy following cultivation for 5 days in both the standard medium containing serum and the serum - free medium ... microscope and representative photo- graphs taken . RESULTS Morphology of Cultured Explants For each medium , at a ...
... microscopy following cultivation for 5 days in both the standard medium containing serum and the serum - free medium ... microscope and representative photo- graphs taken . RESULTS Morphology of Cultured Explants For each medium , at a ...
Page 537
... microscopy of , 209 , 214–217 preparation of tissue for , 206 equipment for , 203 hydration of tissue in , prevention of , 218 materials and reagents for , 202–203 media for , 203 in medium containing serum alternatives to , 218 ...
... microscopy of , 209 , 214–217 preparation of tissue for , 206 equipment for , 203 hydration of tissue in , prevention of , 218 materials and reagents for , 202–203 media for , 203 in medium containing serum alternatives to , 218 ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml