In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 154
... observed in our primary myocardial cell cultures were sim- ilar in nature to the granules and cytoplasmic inclusions observed in the autopsy findings remains to be determined . These data illustrate that in our controlled environment of ...
... observed in our primary myocardial cell cultures were sim- ilar in nature to the granules and cytoplasmic inclusions observed in the autopsy findings remains to be determined . These data illustrate that in our controlled environment of ...
Page 157
... observed ; Fib , contractile activity was irregular , asynchronous , and / or arrhythmic . Percentages of areas which exhibited the specified beating rate are given in parentheses . Values are expressed as the mean beats / min ± S.E.M. ...
... observed ; Fib , contractile activity was irregular , asynchronous , and / or arrhythmic . Percentages of areas which exhibited the specified beating rate are given in parentheses . Values are expressed as the mean beats / min ± S.E.M. ...
Page 185
... observed ( 2 ) . Cell Proliferation The population doubling time of the predominant epithelial cells is 26 hr . The mitotic index is maximum on day 2 ( 2.0 % ) , and colchicine increases this value to 5.4 % ( 2 ) . Subculture Monolayers ...
... observed ( 2 ) . Cell Proliferation The population doubling time of the predominant epithelial cells is 26 hr . The mitotic index is maximum on day 2 ( 2.0 % ) , and colchicine increases this value to 5.4 % ( 2 ) . Subculture Monolayers ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml