In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 37
... obtained either by us- ing computer - assisted imaging techniques from photomicrographs of the sec- tions as previously described ( 21 ) or by processing images directly from video cameras viewing the sections through the microscope at ...
... obtained either by us- ing computer - assisted imaging techniques from photomicrographs of the sec- tions as previously described ( 21 ) or by processing images directly from video cameras viewing the sections through the microscope at ...
Page 148
... obtained from offspring of the swim - trained dams were less sensitive to the toxic effects of ethanol than those obtained from the offspring of the seden- tary dams ( 4,5 ) . Interestingly , maternal diet - stress during pregnancy also ...
... obtained from offspring of the swim - trained dams were less sensitive to the toxic effects of ethanol than those obtained from the offspring of the seden- tary dams ( 4,5 ) . Interestingly , maternal diet - stress during pregnancy also ...
Page 417
... obtained . This limitation is highlighted in work by Hottendorf ( 20 ) , who reported that the relatively nonnephrotoxic cephalosporin antibiotic cephalothin was highly toxic to LLC - RK , and LLC - PK , cells . Hottendorf and co ...
... obtained . This limitation is highlighted in work by Hottendorf ( 20 ) , who reported that the relatively nonnephrotoxic cephalosporin antibiotic cephalothin was highly toxic to LLC - RK , and LLC - PK , cells . Hottendorf and co ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml