In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 235
... plastic beaker by means of a plastic or stainless steel cylinder with semicircular openings cut out at the base ( to avoid any squeezing of cell suspension through narrow slits ) . Plastic beakers : Disposable , 150 - ml plastic beakers ...
... plastic beaker by means of a plastic or stainless steel cylinder with semicircular openings cut out at the base ( to avoid any squeezing of cell suspension through narrow slits ) . Plastic beakers : Disposable , 150 - ml plastic beakers ...
Page 264
... Plastic beaker ( 4 liters ) Two large ( 2 - liter ) plastic bowls ( 2 to 4 inch rim ) High - capacity peristaltic pump ( up to 1140 ml / min ) Büchner funnel ( 10-12 inches ) Fritted glass tube Biopsy perfusion supplies : Beaker ( glass ...
... Plastic beaker ( 4 liters ) Two large ( 2 - liter ) plastic bowls ( 2 to 4 inch rim ) High - capacity peristaltic pump ( up to 1140 ml / min ) Büchner funnel ( 10-12 inches ) Fritted glass tube Biopsy perfusion supplies : Beaker ( glass ...
Page 308
... plastic may reflect the behavior of lipocytes in liver injury . The state of activation induced by cul- ture must therefore be considered in designing experiments to test the response of lipocytes to cytokines or pharmocologic agents ...
... plastic may reflect the behavior of lipocytes in liver injury . The state of activation induced by cul- ture must therefore be considered in designing experiments to test the response of lipocytes to cytokines or pharmocologic agents ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml