In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 317
... populations are heterogeneous and contain cells with different de- velopmental potential and at various stages of differentiation and lineage com- mitment . Uncloned lines obtained from these populations usually reflect this ...
... populations are heterogeneous and contain cells with different de- velopmental potential and at various stages of differentiation and lineage com- mitment . Uncloned lines obtained from these populations usually reflect this ...
Page 397
... Populations by Percoll Density - Gradient Centrifugation Lawrence H. Lash INTRODUCTION The basic structural unit of the mammalian kidney , the nephron , is composed of several cell populations , each exhibiting characteristic morphology ...
... Populations by Percoll Density - Gradient Centrifugation Lawrence H. Lash INTRODUCTION The basic structural unit of the mammalian kidney , the nephron , is composed of several cell populations , each exhibiting characteristic morphology ...
Page 401
... Populations and Asssessment of Purity To identify the two cell populations , the distribution of marker enzyme activi- ties on the gradient is measured ( Fig . 1B ) . Alkaline phosphatase ( EC 3.1.3.1 ) and y - glutamyltransferase ( EC ...
... Populations and Asssessment of Purity To identify the two cell populations , the distribution of marker enzyme activi- ties on the gradient is measured ( Fig . 1B ) . Alkaline phosphatase ( EC 3.1.3.1 ) and y - glutamyltransferase ( EC ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml