In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 172
... preparation to perfused segments by Lewis and Koessler in 1927 in pig carotid arteries ( 14 ) , and it became a ... preparation ( 18,19 ) . Regardless , it is critical to develop a method of preparation for spiral strips that is ...
... preparation to perfused segments by Lewis and Koessler in 1927 in pig carotid arteries ( 14 ) , and it became a ... preparation ( 18,19 ) . Regardless , it is critical to develop a method of preparation for spiral strips that is ...
Page 196
... prepared and kept frozen until needed for solution preparation . On the day of the experiment , 50 ml of Hanks ' solution is prepared by mixing the following stock solutions with 22 ml water : 5 ml of 10 × Hanks ' , 5 ml HEPES , 5 ml ...
... prepared and kept frozen until needed for solution preparation . On the day of the experiment , 50 ml of Hanks ' solution is prepared by mixing the following stock solutions with 22 ml water : 5 ml of 10 × Hanks ' , 5 ml HEPES , 5 ml ...
Page 540
... preparation of , 50–51 preparation of reaggregates from , 27 removal of , 31–32 Embryonic cells , preparation of , for reag- gregate cultures , 30-31 Embryonic growth , in culture , evaluation of , 427-428 scoring system for , 429 ...
... preparation of , 50–51 preparation of reaggregates from , 27 removal of , 31–32 Embryonic cells , preparation of , for reag- gregate cultures , 30-31 Embryonic growth , in culture , evaluation of , 427-428 scoring system for , 429 ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml