In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 251
... produce liver allergic reactions ( 17,18,21,23 ) . Ex- amples of these hepatotoxicants are acetaminophen , carbon ... produced by the whole animal ( 21,23,24 ) . For example , liver cell cul- tures treated with papaverine hydrochloride ...
... produce liver allergic reactions ( 17,18,21,23 ) . Ex- amples of these hepatotoxicants are acetaminophen , carbon ... produced by the whole animal ( 21,23,24 ) . For example , liver cell cul- tures treated with papaverine hydrochloride ...
Page 279
... produce , a significant effort has been made to develop in vitro systems that can adequately simulate events that ... produced by the parent form of the compound . Historically , several in vitro approaches have been utilized to study ...
... produce , a significant effort has been made to develop in vitro systems that can adequately simulate events that ... produced by the parent form of the compound . Historically , several in vitro approaches have been utilized to study ...
Page 409
... produce cytotoxicity , as tBH produced the same degree of GSH depletion in both PT and DT cells and yet was markedly more cytotoxic in DT cells ( 18 ) . To study the susceptibility of isolated PT and DT cells to alkylation , the ef ...
... produce cytotoxicity , as tBH produced the same degree of GSH depletion in both PT and DT cells and yet was markedly more cytotoxic in DT cells ( 18 ) . To study the susceptibility of isolated PT and DT cells to alkylation , the ef ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml