In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 59
... protocols and then reserve a one - year's supply of the selected lot . Considerable effort has been directed to replacing serum with defined for- mulations of growth factors , hormones , and specific nutrients in other neuronal culture ...
... protocols and then reserve a one - year's supply of the selected lot . Considerable effort has been directed to replacing serum with defined for- mulations of growth factors , hormones , and specific nutrients in other neuronal culture ...
Page 321
... Protocols have been optimized for oval cell and parenchymal cell isolation separately . It has been our experience that it is ... protocol for oval cell isolation utilizes both collagenase and Pronase which are prepared as separate stock ...
... Protocols have been optimized for oval cell and parenchymal cell isolation separately . It has been our experience that it is ... protocol for oval cell isolation utilizes both collagenase and Pronase which are prepared as separate stock ...
Page 446
... protocol , calculate the amount of media needed to change all plates / flasks ( using the figures in the tabulation above table multiplied by the number of plates plus an additional 5 % " cushion " ) . Table I lists the supplements and ...
... protocol , calculate the amount of media needed to change all plates / flasks ( using the figures in the tabulation above table multiplied by the number of plates plus an additional 5 % " cushion " ) . Table I lists the supplements and ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml