In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 345
... proximal tubules ( 6 ) , the DME - F12 was supplemented with heptanoic acid to provide an ade- quate nutrient supply to support oxidative metabolism over the 24 - hr period . In contrast to monolayer cultures of proximal tubules which ...
... proximal tubules ( 6 ) , the DME - F12 was supplemented with heptanoic acid to provide an ade- quate nutrient supply to support oxidative metabolism over the 24 - hr period . In contrast to monolayer cultures of proximal tubules which ...
Page 371
... proximal tubule cells is highly reproducible and yields a large number of cells . As many as 100 35 - mm dishes containing confluent monolayers can be obtained from a single rabbit kidney . Moreover the cultures express functional ...
... proximal tubule cells is highly reproducible and yields a large number of cells . As many as 100 35 - mm dishes containing confluent monolayers can be obtained from a single rabbit kidney . Moreover the cultures express functional ...
Page 555
... proximal tubule culture , 341 Pentobarbital - heparin solution , 126 PEPCK . See Phosphoenolpyruvate car- boxykinase Percoll density - gradient centrifugation , 113 continuous , isolation of renal proximal tubules with procedures for ...
... proximal tubule culture , 341 Pentobarbital - heparin solution , 126 PEPCK . See Phosphoenolpyruvate car- boxykinase Percoll density - gradient centrifugation , 113 continuous , isolation of renal proximal tubules with procedures for ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml