In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 33
... reaggregates are allowed to settle by gravitation . All but 0.5 ml of the medium is removed and replaced with 3.0 ml fresh medium containing horse serum instead of ... Reaggregates serving as controls receive an 3. Reaggregate Cultures 33.
... reaggregates are allowed to settle by gravitation . All but 0.5 ml of the medium is removed and replaced with 3.0 ml fresh medium containing horse serum instead of ... Reaggregates serving as controls receive an 3. Reaggregate Cultures 33.
Page 42
... Reaggregates are formed and allowed to develop for 15 days . At that time , the following three groups of reaggregate flasks with six replicates in each group are prepared by pooling and redistribution : ( 1 ) control flasks in which ...
... Reaggregates are formed and allowed to develop for 15 days . At that time , the following three groups of reaggregate flasks with six replicates in each group are prepared by pooling and redistribution : ( 1 ) control flasks in which ...
Page 43
... reaggregates are exposed to the putative protective agent , alone , at the highest concentration in order to control for neurotoxic or other effects ... reaggregates by catecholamine - induced histofluorescence as 3. Reaggregate Cultures 43.
... reaggregates are exposed to the putative protective agent , alone , at the highest concentration in order to control for neurotoxic or other effects ... reaggregates by catecholamine - induced histofluorescence as 3. Reaggregate Cultures 43.
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml