In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 68
... requires considerably less tissue and has several orders of magnitude higher temporal resolution than most biochemical ... require special skills and equipment . ACKNOWLEGMENTS Supported by a grant from The Johns Hopkins University ...
... requires considerably less tissue and has several orders of magnitude higher temporal resolution than most biochemical ... require special skills and equipment . ACKNOWLEGMENTS Supported by a grant from The Johns Hopkins University ...
Page 240
... require considerable skill and practice , and they may be the quality - limiting step for the beginner . Three factors seem to be particu- larly problematic . The maintenance of light ether anesthesia requires continuous attention to ...
... require considerable skill and practice , and they may be the quality - limiting step for the beginner . Three factors seem to be particu- larly problematic . The maintenance of light ether anesthesia requires continuous attention to ...
Page 409
... require complicated machinery or de- tailed surgical methods ; they yield an ample supply of high - purity cells in only 3.5 hr , and the cells can be used to study detailed biochemical mechanisms of chemical cytotoxicity . The PT and ...
... require complicated machinery or de- tailed surgical methods ; they yield an ample supply of high - purity cells in only 3.5 hr , and the cells can be used to study detailed biochemical mechanisms of chemical cytotoxicity . The PT and ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml