In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 108
... separate the anterior from the posterior segment . The posterior segment ( eyecup ) is soaked in medium for 1-2 min , which expands the neural retina . The absence of any pig- mented spots on the neural retina as it is removed indicates ...
... separate the anterior from the posterior segment . The posterior segment ( eyecup ) is soaked in medium for 1-2 min , which expands the neural retina . The absence of any pig- mented spots on the neural retina as it is removed indicates ...
Page 397
... separate different renal cell populations from one another . Differences in surface charge have been used in electrophoretic methods to separate proximal tubular ( PT ) and distal tubular ( DT ) cells from rabbit ( 3 ) and rat ( 4 ) ...
... separate different renal cell populations from one another . Differences in surface charge have been used in electrophoretic methods to separate proximal tubular ( PT ) and distal tubular ( DT ) cells from rabbit ( 3 ) and rat ( 4 ) ...
Page 563
... separate regions of , 104 11 - cis - Retinaldehyde binding protein , 106 Retinal pigment epithelium , 100 cultured , biochemistry of , 105–106 culture of , 100–109 foveal and peripheral , functional capac- ities of , 104 functional ...
... separate regions of , 104 11 - cis - Retinaldehyde binding protein , 106 Retinal pigment epithelium , 100 cultured , biochemistry of , 105–106 culture of , 100–109 foveal and peripheral , functional capac- ities of , 104 functional ...
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml