In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 5
... Slices cut by vibratome will consist of hippocampus embedded in other tissue . These slices can be used as is , or the hippocampal portion can be dissected free . This and other microdissection pro- cedures ( such as isolating certain ...
... Slices cut by vibratome will consist of hippocampus embedded in other tissue . These slices can be used as is , or the hippocampal portion can be dissected free . This and other microdissection pro- cedures ( such as isolating certain ...
Page 222
... slices provides certain advantages and thus is considered a valuable addition to currently available in vitro methodologies . This brief introduction focuses on the development of preci- sion - cut liver slice technology and the ...
... slices provides certain advantages and thus is considered a valuable addition to currently available in vitro methodologies . This brief introduction focuses on the development of preci- sion - cut liver slice technology and the ...
Page 224
... slices . Slices are produced by mechanically pulling the immobilized , weighted tissue cylinder across a rapidly oscillating razor blade ( E ) that operates ... slice culture medium A B roller vial с heated rollers 224 VIA . Liver Slices.
... slices . Slices are produced by mechanically pulling the immobilized , weighted tissue cylinder across a rapidly oscillating razor blade ( E ) that operates ... slice culture medium A B roller vial с heated rollers 224 VIA . Liver Slices.
Other editions - View all
In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml