In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 150
... Step 1 MINCING OF HEART Step 2 POOLING HEART FRAGMENTS Step 3 Step 10 DRUG ADMINISTRATION & TOXICITY TESTING CELL INCUBATION FOR 4-5 DAYS Step 8 PLATING CELLS IN Step 7 TRYPSINIZATION CELL ISOLATION Step 4 CENTRIFUGATION CELL WASHING Step 5 ...
... Step 1 MINCING OF HEART Step 2 POOLING HEART FRAGMENTS Step 3 Step 10 DRUG ADMINISTRATION & TOXICITY TESTING CELL INCUBATION FOR 4-5 DAYS Step 8 PLATING CELLS IN Step 7 TRYPSINIZATION CELL ISOLATION Step 4 CENTRIFUGATION CELL WASHING Step 5 ...
Page 231
... step procedure . In the first step , the liver is subjected to a nonrecirculating perfusion with calcium- free buffer or with a calcium chelator like EGTA , causing irreversible separation of desmosomal cell contacts . In the second ...
... step procedure . In the first step , the liver is subjected to a nonrecirculating perfusion with calcium- free buffer or with a calcium chelator like EGTA , causing irreversible separation of desmosomal cell contacts . In the second ...
Page 242
... step . If , for some reason , recirculating perfusion is used for this first step , a Ca2 + chelator like EGTA should be included . If repeated cell prepara- tions are made using recirculation at both steps and the apparatus is not ade ...
... step . If , for some reason , recirculating perfusion is used for this first step , a Ca2 + chelator like EGTA should be included . If repeated cell prepara- tions are made using recirculation at both steps and the apparatus is not ade ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml