In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 247
... sterile filtering unit housing a Gelman ( Ann Arbor , MI ) metrical GA - B membrane filter ( 0.2 μm / 90 mm ) . The sterile filtering unit is connected to the dispens- ing pressure vessel and to a sterile 1000 - ml Erlenmeyer flask ( to ...
... sterile filtering unit housing a Gelman ( Ann Arbor , MI ) metrical GA - B membrane filter ( 0.2 μm / 90 mm ) . The sterile filtering unit is connected to the dispens- ing pressure vessel and to a sterile 1000 - ml Erlenmeyer flask ( to ...
Page 368
... sterilized , dispensed into 20 - ml aliquots in sterile 50 - ml flasks , and stored at -20 ° C . 6. One liter of a sterile solution of 0.25 % trypsin / 1 mM EDTA in PBS is used in the trypsinization of primary proximal tubule cell ...
... sterilized , dispensed into 20 - ml aliquots in sterile 50 - ml flasks , and stored at -20 ° C . 6. One liter of a sterile solution of 0.25 % trypsin / 1 mM EDTA in PBS is used in the trypsinization of primary proximal tubule cell ...
Page 369
... sterile scissors , kidneys with renal arteries and veins intact are removed . The kidneys are placed in a sterile 50 - ml tube containing DME / F12 ( at 4 ° C ) with 192 IU / ml penicillin and 200 μg / ml streptomycin . Tubes containing ...
... sterile scissors , kidneys with renal arteries and veins intact are removed . The kidneys are placed in a sterile 50 - ml tube containing DME / F12 ( at 4 ° C ) with 192 IU / ml penicillin and 200 μg / ml streptomycin . Tubes containing ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml