In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page 1
... synaptic elements make the hippocampus particularly well suited for this type of investigation . The large body of knowledge about this brain region further recommends the hippocam- pal slice to investigators studying the mechanisms of ...
... synaptic elements make the hippocampus particularly well suited for this type of investigation . The large body of knowledge about this brain region further recommends the hippocam- pal slice to investigators studying the mechanisms of ...
Page 27
... synaptic connections ( 13 ) . The nature of such connections can be specified by the types of cells in- cluded in the flask prior to reaggregation . Of particular interest are reaggregates prepared from cells from a region of the brain ...
... synaptic connections ( 13 ) . The nature of such connections can be specified by the types of cells in- cluded in the flask prior to reaggregation . Of particular interest are reaggregates prepared from cells from a region of the brain ...
Page 70
... synaptic events . Given their neural crest origin , chromaffin cells are analogous to sympathetic ganglia . The use of these cells in research has also expanded our understanding of the electrophysiology of ion - channel conduc- tances ...
... synaptic events . Given their neural crest origin , chromaffin cells are analogous to sympathetic ganglia . The use of these cells in research has also expanded our understanding of the electrophysiology of ion - channel conduc- tances ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml