In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Page xvii
... volume in this series covering techniques for preparation and maintenance of in vitro cell and tissue systems for toxicological studies . The- matic material in this and future volumes will focus on mechanistic and risk as- sessment ...
... volume in this series covering techniques for preparation and maintenance of in vitro cell and tissue systems for toxicological studies . The- matic material in this and future volumes will focus on mechanistic and risk as- sessment ...
Page 37
... volume of the jth section and y is the same density parameter as that for the whole flask . In that case , the maximum likelihood estimate , m , for y is given by 30 m = ΣΝ . Σα and the estimate for vy , the expected number of ...
... volume of the jth section and y is the same density parameter as that for the whole flask . In that case , the maximum likelihood estimate , m , for y is given by 30 m = ΣΝ . Σα and the estimate for vy , the expected number of ...
Page 53
... volume in each well down to 125 μl and adding 750 μl of HCSS with a repeat pipettor . This step is repeated a second time ; then toxic exposure is initiated by aspirating the volume down to 125 μl and pipetting back 250 μl of HCSS plus ...
... volume in each well down to 125 μl and adding 750 μl of HCSS with a repeat pipettor . This step is repeated a second time ; then toxic exposure is initiated by aspirating the volume down to 125 μl and pipetting back 250 μl of HCSS plus ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Limited preview - 2016 |
Common terms and phrases
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml