Physical Principles and Techniques of Protein Chemistry, Part 1

absorbance absorption acid amino appears applied atom axis binding birefringence boundary buffer calculated cell charge Chem chromophores complex concentration conformational constant containing corrected corresponding curve decrease dependence determined dielectric difference diffusion dipole direction discussed effect electric electric field electron electrophoresis emission emission spectrum energy equation equilibrium example excitation experimental experiments factor fluorescence fraction frequency function given groups important increase indicates intensity interactions ionic ions light limited macromolecules measured method mobility molecular molecules observed obtained occurs optical orientation particles patterns peaks perturbation phase phenolic polarization position possible present produced protein quantum yield ratio reaction reference relative relaxation residues respectively rotation sample separation serum albumin shift shown single solution solvent spectra spectrum strength structure studies technique temperature theory tion transfer transition tryptophan tyrosine unit usually values volume wavelength Weber zone