Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 118
... concentration , in which all the chromophores are either ion- ized or un - ionized . Choosing the reference solution of un - ionized chro- mophores at the same concentration ( pH fixed and a = 0 ) Dref ( c , X ) = EUC If the molar ...
... concentration , in which all the chromophores are either ion- ized or un - ionized . Choosing the reference solution of un - ionized chro- mophores at the same concentration ( pH fixed and a = 0 ) Dref ( c , X ) = EUC If the molar ...
Page 473
... Concentration ( mg / ml ) FIG . 7. The elution volume of sulfatase A as a function of protein concentration . The various solutions of enzyme were applied to a 1.5 X 50 cm column of Sephadex G - 200 equilibrated with uniunivalent ...
... Concentration ( mg / ml ) FIG . 7. The elution volume of sulfatase A as a function of protein concentration . The various solutions of enzyme were applied to a 1.5 X 50 cm column of Sephadex G - 200 equilibrated with uniunivalent ...
Page 475
... concentration dependence of migration rate ( Winzor and Nichol , 1965 ) . Consequently , estimates of solute purity require analyses whereby effects of concentration dependence may be either taken into account or neglected . A ...
... concentration dependence of migration rate ( Winzor and Nichol , 1965 ) . Consequently , estimates of solute purity require analyses whereby effects of concentration dependence may be either taken into account or neglected . A ...
Contents
SLAYTER | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shadow shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone