Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 131
... conformational changes in most proteins studied under reasonable conditions of pH , ionic strength , and temperature . Conformational changes can be suspected if the perturbation is found to be nonlinear with respect to perturbant ...
... conformational changes in most proteins studied under reasonable conditions of pH , ionic strength , and temperature . Conformational changes can be suspected if the perturbation is found to be nonlinear with respect to perturbant ...
Page 146
... conformational change , or on a surface at which association takes place , then an artificial chromophore may be inserted by chemical modification . of the protein . Such a chromophore has been termed a " reporter group " ( Burr and ...
... conformational change , or on a surface at which association takes place , then an artificial chromophore may be inserted by chemical modification . of the protein . Such a chromophore has been termed a " reporter group " ( Burr and ...
Page 226
... conformational changes in proteins . The optical methods of rotatory dispersion , circular dichroism , difference spectra , and fluorescence meas- urements offer many advantages in terms of sensitivity , specificity , and kinetic ...
... conformational changes in proteins . The optical methods of rotatory dispersion , circular dichroism , difference spectra , and fluorescence meas- urements offer many advantages in terms of sensitivity , specificity , and kinetic ...
Contents
SLAYTER | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shadow shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone