Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 222
... containing several tyrosyl residues . Polarization values which were smaller than those of tyrosine were observed in ribonuclease , insulin , and zein at -70 ° C in propylene glycol ( Weber , 1960b ) . The excitation polarization ...
... containing several tyrosyl residues . Polarization values which were smaller than those of tyrosine were observed in ribonuclease , insulin , and zein at -70 ° C in propylene glycol ( Weber , 1960b ) . The excitation polarization ...
Page 276
... containing two small ( about 0.5 ml ) cylindrical vessels equipped with electrodes . One of these cells contains the test solution , and the other is a reference cell containing solvent . The difference in conductivity between the ...
... containing two small ( about 0.5 ml ) cylindrical vessels equipped with electrodes . One of these cells contains the test solution , and the other is a reference cell containing solvent . The difference in conductivity between the ...
Page 410
... containing acetate buffer ( Timasheff , 1967 ) . B - Lactoglobulin in par- ticularly interesting since it differs from most other proteins in that its electrophoretic behavior reveals protein - acetate buffer interaction at pH values ...
... containing acetate buffer ( Timasheff , 1967 ) . B - Lactoglobulin in par- ticularly interesting since it differs from most other proteins in that its electrophoretic behavior reveals protein - acetate buffer interaction at pH values ...
Contents
SLAYTER | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shadow shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone