Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
From inside the book
Results 1-3 of 90
Page 78
... effect in L - alanine is given by Marsh and Donohue ( 1967 ) . The effect of disorder on a Fourier synthesis is illustrated in Fig . 13 and it will be seen that an increase in B leads to a reduction in the effective resolution . In ...
... effect in L - alanine is given by Marsh and Donohue ( 1967 ) . The effect of disorder on a Fourier synthesis is illustrated in Fig . 13 and it will be seen that an increase in B leads to a reduction in the effective resolution . In ...
Page 127
... effects would then take the same path from the a - carbon atom . In agree- ment with this hypothesis , the ratio of the effect of deprotonation of the carboxyl group to the effect of deprotonation of the NH3 + group appears to be ...
... effects would then take the same path from the a - carbon atom . In agree- ment with this hypothesis , the ratio of the effect of deprotonation of the carboxyl group to the effect of deprotonation of the NH3 + group appears to be ...
Page 128
... effect mediated by the solvent proper and therefore affected by ionic strength . ( 5 ) An inductive effect through the covalent bonds of the protein . ( 6 ) An alteration in the water struc- ture ( electrostrictive changes ) near the ...
... effect mediated by the solvent proper and therefore affected by ionic strength . ( 5 ) An inductive effect through the covalent bonds of the protein . ( 6 ) An alteration in the water struc- ture ( electrostrictive changes ) near the ...
Contents
SLAYTER | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
58 other sections not shown
Other editions - View all
Common terms and phrases
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shadow shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone