Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 193
... emission , Raman lines , the photosensitivity of the fluorescent compound , or background electrical noise . Filter ... spectrum , the distribution of quanta q emitted at all wavelengths à is determined by a monochromator . Ideally , the ...
... emission , Raman lines , the photosensitivity of the fluorescent compound , or background electrical noise . Filter ... spectrum , the distribution of quanta q emitted at all wavelengths à is determined by a monochromator . Ideally , the ...
Page 194
... emission spectra which deviate from . true spectra due to the wavelength - dependent variation of detector re- sponse and photon output of the light source . Since no two instruments . are exactly alike , apparent spectra obtained with ...
... emission spectra which deviate from . true spectra due to the wavelength - dependent variation of detector re- sponse and photon output of the light source . Since no two instruments . are exactly alike , apparent spectra obtained with ...
Page 216
... emission spectra of molecules of biochemical interest have appeared . The ... spectrum of indole 30 mμ toward the red compared to the peak in pure ... emission spectra of the tyrosyl residue in polypeptides are little affected by solvent ...
... emission spectra of molecules of biochemical interest have appeared . The ... spectrum of indole 30 mμ toward the red compared to the peak in pure ... emission spectra of the tyrosyl residue in polypeptides are little affected by solvent ...
Contents
SLAYTER | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shadow shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone