Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
From inside the book
Results 1-3 of 48
Page 186
... excitation and emission oscillators to approach its random value and hence to reduce the magnitude of the ... Excitation Polarization Spectra The value of P. , the limiting polarization in the absence of Brownian rotation , is a function ...
... excitation and emission oscillators to approach its random value and hence to reduce the magnitude of the ... Excitation Polarization Spectra The value of P. , the limiting polarization in the absence of Brownian rotation , is a function ...
Page 193
... excitation and emission although very broad ( 40 mp ) bandwidths are used , but in the filter fluorometer there is considerable overlap in transmission characteristics of the most suit- able primary and secondary filters . In the case ...
... excitation and emission although very broad ( 40 mp ) bandwidths are used , but in the filter fluorometer there is considerable overlap in transmission characteristics of the most suit- able primary and secondary filters . In the case ...
Page 194
... excitation and emission spectra which deviate from . true spectra due to the wavelength - dependent variation of detector re- sponse and photon output of the light source . Since no two instruments . are exactly alike , apparent spectra ...
... excitation and emission spectra which deviate from . true spectra due to the wavelength - dependent variation of detector re- sponse and photon output of the light source . Since no two instruments . are exactly alike , apparent spectra ...
Contents
SLAYTER | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
58 other sections not shown
Other editions - View all
Common terms and phrases
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shadow shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone