Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 384
... moving boundary is an interface between , in this case , an albumin solution and a buffer con- taining no albumin , the protein is said to disappear across the boundary . The electrophoretic patterns of a complex mixture are well ...
... moving boundary is an interface between , in this case , an albumin solution and a buffer con- taining no albumin , the protein is said to disappear across the boundary . The electrophoretic patterns of a complex mixture are well ...
Page 391
... moving boundary is also accompanied by ad- justments in the concentration of all other ionic species that may be present . Consequently , the change in refractive index across the moving boundary , and hence the area under the peak in ...
... moving boundary is also accompanied by ad- justments in the concentration of all other ionic species that may be present . Consequently , the change in refractive index across the moving boundary , and hence the area under the peak in ...
Page 421
... moving - boundary electrophoresis . Nor is zone electrophoresis immune to the several nonideal effects which sometimes complicate moving - boundary electrophoresis . Recent theoretical and experimental developments make it clear that ...
... moving - boundary electrophoresis . Nor is zone electrophoresis immune to the several nonideal effects which sometimes complicate moving - boundary electrophoresis . Recent theoretical and experimental developments make it clear that ...
Contents
SLAYTER | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shadow shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone