Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 153
... Photomultipliers will be noisier when the high voltage is first applied to them , and they will exhibit some hys ... photomultiplier dynode voltage ( or slit program ) should be adjusted to the setting de- sired for subsequent use ...
... Photomultipliers will be noisier when the high voltage is first applied to them , and they will exhibit some hys ... photomultiplier dynode voltage ( or slit program ) should be adjusted to the setting de- sired for subsequent use ...
Page 212
... photomultiplier tubes , so that two modulated photocurrents are produced . Müller et al . ( 1965 ) have discussed the features desirable in the photomultiplier . The output from the photomultiplier monitoring the sample cuvette enters a ...
... photomultiplier tubes , so that two modulated photocurrents are produced . Müller et al . ( 1965 ) have discussed the features desirable in the photomultiplier . The output from the photomultiplier monitoring the sample cuvette enters a ...
Page 283
... photomultiplier side of the flow tube instead of in the usual position directly in front of the lamp ( Chance ... photomultiplier ) Turbidity Overlapping spectra ( poor selectivity ) Lamp instability Poor optical alignment Stray light ...
... photomultiplier side of the flow tube instead of in the usual position directly in front of the lamp ( Chance ... photomultiplier ) Turbidity Overlapping spectra ( poor selectivity ) Lamp instability Poor optical alignment Stray light ...
Contents
SLAYTER | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shadow shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone