Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
From inside the book
Results 1-3 of 89
Page 136
... produced by fluorescence . Perturbation difference spectra of N - acetyl - L - tyrosine ethyl ester and N - acetyl - L - tryptophan ethyl ester produced by the perturbing solvents D2O , methanol , dimethyl sulfoxide , ethylene glycol ...
... produced by fluorescence . Perturbation difference spectra of N - acetyl - L - tyrosine ethyl ester and N - acetyl - L - tryptophan ethyl ester produced by the perturbing solvents D2O , methanol , dimethyl sulfoxide , ethylene glycol ...
Page 139
... produced by the protein interior with the perturbation produced by 20 % ethylene glycol indicates that the interior of a protein is equivalent to 120 % ethylene glycol . Esti- mates of Ae for release of buried chromophores are given in ...
... produced by the protein interior with the perturbation produced by 20 % ethylene glycol indicates that the interior of a protein is equivalent to 120 % ethylene glycol . Esti- mates of Ae for release of buried chromophores are given in ...
Page 141
... produced by ethylene glycol ( Fig . 9 ) . The magnitude ( height ) of the difference spec- trum has the same pH dependence as the activity of the enzyme . When lysozyme is denatured with increasing concentrations of urea , the height ...
... produced by ethylene glycol ( Fig . 9 ) . The magnitude ( height ) of the difference spec- trum has the same pH dependence as the activity of the enzyme . When lysozyme is denatured with increasing concentrations of urea , the height ...
Contents
SLAYTER | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
58 other sections not shown
Other editions - View all
Common terms and phrases
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shadow shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone