Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
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Page 421
... zone electrophoretic patterns as moving - boundary patterns . In particular , cognizance must be taken of the fact that multiple zones need not necessarily indicate inherent heterogeneity . As will be shown below , a single ...
... zone electrophoretic patterns as moving - boundary patterns . In particular , cognizance must be taken of the fact that multiple zones need not necessarily indicate inherent heterogeneity . As will be shown below , a single ...
Page 437
Sydney J. Leach. migrated in the slower zone when reanalyzed ( Fig . 26d ) . This result underscores the conclusion that the two zones arise from interaction . Zone electrophoresis of bovine serum albumin has also been carried out in ...
Sydney J. Leach. migrated in the slower zone when reanalyzed ( Fig . 26d ) . This result underscores the conclusion that the two zones arise from interaction . Zone electrophoresis of bovine serum albumin has also been carried out in ...
Page 438
... zone patterns . While in certain instances the generation . of multiple zones due to interaction may be relatively insensitive to protein concentration [ for example , bovine serum albumin in phosphate- borate buffer ( pH 6.1 ) ] , in ...
... zone patterns . While in certain instances the generation . of multiple zones due to interaction may be relatively insensitive to protein concentration [ for example , bovine serum albumin in phosphate- borate buffer ( pH 6.1 ) ] , in ...
Contents
SLAYTER | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
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absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration contrast curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis electrophoretic patterns elution volume emission energy enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups heavy atom intensity interactions ionic strength ionization ions light macromolecules measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer protein proton quantum yield ratio reaction relaxation residues resolution ribonuclease shadow shown in Fig solution solvent specimen spectra structure technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone