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acid addition amount angle appears applied band beam becomes calculated Chem compared complex component concentration conformation constant containing contribution corresponding creatine cross-section density dependence described determined diffusible dilute discussed effect electron electron density enzyme equation equilibrium et al example experimental experiments factor field function given gives grams gyration increase indicated intensity interaction known Kratky length magnetic mass means measurements membrane method mole molecular weight molecule negative observed obtained optical activity parameters particles plot positive possible pressure procedure protein solution radius of gyration range ratio reference region relative residues respectively rotational strength sample scattering curve shape shown shows side similar small-angle solvent specific volume spectra spectrum sphere structure studies subunits Table temperature theoretical theory tion transition usually various varying wavelength X-ray zero