Physical Principles and Techniques of Protein Chemistry, Part 3Sydney J. Leach |
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Page 188
... buffer is by no means sufficient ( as is sometimes thought ) , since for small - angle scat- tering the difference in electron densities is decisive . In every case , it is best to dialyze the protein solution against the buffer used as ...
... buffer is by no means sufficient ( as is sometimes thought ) , since for small - angle scat- tering the difference in electron densities is decisive . In every case , it is best to dialyze the protein solution against the buffer used as ...
Page 274
... Buffer The buffer or other supporting medium should be such that there is minimal interaction with Mn2 + . The metal ion is normally at a concen- tration of the order of 10-4 M and as the buffer concentration is expected to be greater ...
... Buffer The buffer or other supporting medium should be such that there is minimal interaction with Mn2 + . The metal ion is normally at a concen- tration of the order of 10-4 M and as the buffer concentration is expected to be greater ...
Page 276
... Buffer system 1.600 2.256 0.44 Buffer system + MnCl2 ( 1.2 × 10-4 M ) 0.520 Buffer system + MnCl2 + PEPCK 0.733 1.36 0.92 0.252 0.355 2.82 2.38 2.59 ( 0.71 X 10-4 M ) Buffer system + MnCl2 + PEPCK 0.150 0.212 4.72 4.28 4.65 ( 1.48 × 10 ...
... Buffer system 1.600 2.256 0.44 Buffer system + MnCl2 ( 1.2 × 10-4 M ) 0.520 Buffer system + MnCl2 + PEPCK 0.733 1.36 0.92 0.252 0.355 2.82 2.38 2.59 ( 0.71 X 10-4 M ) Buffer system + MnCl2 + PEPCK 0.150 0.212 4.72 4.28 4.65 ( 1.48 × 10 ...
Contents
25 | 4 |
E The Isopotential Specific Volume | 10 |
Direct Volume Change | 22 |
Copyright | |
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a-helical a-helix absorption band acid angle B-structure Beychok Biochem Biochemistry Biol Blout c₂ calculated CD band CD spectrum Chem chromophore circular dichroism coefficient Cohn coil complex concentration conformation contribution Cotton effects creatine kinase cross-section denatured determined dipole Doty electron density ellipticity enzyme equation equilibrium exciton experimental factor Fasman function helical content helix intensity interaction kinase Kratky lysozyme magnetic measurements method Moffitt molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve parameters partial specific volume particles peptide perturbation Phys Pilz plot polarized poly-L-proline polymer polypeptides positive procedure protein protein solution pulse Pysh radius of gyration random coil region residues rotational strength rotatory sample Schellman Section shape small-angle solvent spectra sphere studies subunits T₁ temperature theory Tinoco tion transition transition dipole moment tyrosine vector wavefunctions wavelength Woody X-ray zero