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absorption acid addition angle appears application approximately B-structure band becomes calculated CD spectra Chem compared complex component concentration conformation constant containing contribution corresponding Cotton effects density dependence derived described determined diffusible discussed electron enzyme equation equilibrium estimate et al example experimental experiments expression factor field function given gives helical helix increased indicated intensity interaction length magnetic measurements mechanism method molecular weight molecule negative observed obtained optical activity osmotic parameters partial positive possible pressure procedure protein protein solution quantum mechanical radius range reference region relative residues respectively rotational strength sample scattering curve shape shown shows side similar solution solvent specific volume spectrum structure studies Table temperature theoretical theory tion transition transition dipole moment usually various vary volume wavelength X-ray zero