Physical Principles and Techniques of Protein Chemistry, Part 3Sydney J. Leach |
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Page 93
... ions . On the solvent medium side , the neutrality condition is simply m ' + = m'_ ( 20 ) If Z2 is positive , a greater number of negative than positive diffusible ions are required for electrical neutrality on the protein side , and ...
... ions . On the solvent medium side , the neutrality condition is simply m ' + = m'_ ( 20 ) If Z2 is positive , a greater number of negative than positive diffusible ions are required for electrical neutrality on the protein side , and ...
Page 94
... ions , m , and m per kilogram of water , Eq . ( 11 ) becomes 2 . 1 1 ᎡᎢ T = ln 1 + M1 ( m2 + m + m ) / 1000 1+ M1 ( m ' ++ m ' _ ) / 1000 ( 22 ) 1 = where M1 is the molecular weight of water . The values of the second term in both ...
... ions , m , and m per kilogram of water , Eq . ( 11 ) becomes 2 . 1 1 ᎡᎢ T = ln 1 + M1 ( m2 + m + m ) / 1000 1+ M1 ( m ' ++ m ' _ ) / 1000 ( 22 ) 1 = where M1 is the molecular weight of water . The values of the second term in both ...
Page 96
... ion on the two sides does not necessarily corre- spond to the diffusible ion imbalance . Often , proteins selectively bind ions of a salt . Such bound ions , of course , may not be counted in the Donnan equilibrium . The binding of one kind ...
... ion on the two sides does not necessarily corre- spond to the diffusible ion imbalance . Often , proteins selectively bind ions of a salt . Such bound ions , of course , may not be counted in the Donnan equilibrium . The binding of one kind ...
Contents
25 | 4 |
E The Isopotential Specific Volume | 10 |
Direct Volume Change | 22 |
Copyright | |
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a-helical a-helix absorption band acid angle B-structure Beychok Biochem Biochemistry Biol Blout c₂ calculated CD band CD spectrum Chem chromophore circular dichroism coefficient Cohn coil complex concentration conformation contribution Cotton effects creatine kinase cross-section denatured determined dipole Doty electron density ellipticity enzyme equation equilibrium exciton experimental factor Fasman function helical content helix intensity interaction kinase Kratky lysozyme magnetic measurements method Moffitt molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve parameters partial specific volume particles peptide perturbation Phys Pilz plot polarized poly-L-proline polymer polypeptides positive procedure protein protein solution pulse Pysh radius of gyration random coil region residues rotational strength rotatory sample Schellman Section shape small-angle solvent spectra sphere studies subunits T₁ temperature theory Tinoco tion transition transition dipole moment tyrosine vector wavefunctions wavelength Woody X-ray zero