## Physical Principles and Techniques of Protein Chemistry, Part 3 |

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Page 305

The difference , or deviation , between Fi and Yi will be denoted by 8 ; and the

least - squares criterion seeks to choose values P1 , . . . , Pm for the

of the model function which minimize the weighted sum of squares n S = ) Wi ( F ...

The difference , or deviation , between Fi and Yi will be denoted by 8 ; and the

least - squares criterion seeks to choose values P1 , . . . , Pm for the

**parameters**of the model function which minimize the weighted sum of squares n S = ) Wi ( F ...

Page 317

B . ERRORS IN

minimized , an unbiased estimate of ... is the minimum value of S and m is the

number of independent

...

B . ERRORS IN

**PARAMETER**ESTIMATES When the sum of squares S has beenminimized , an unbiased estimate of ... is the minimum value of S and m is the

number of independent

**parameters**in the fitting function F . The quantity n − m is...

Page 335

184 7040 THE C IN COLUMN 1 OF CARD 10 SHOULD BE REMOVED AND C

INSERTED IN COLUMN 1 OF CARDS 1 AND 11 00000000 0000 o วววว 11 12

13 16 17 18 19 PROGRAM LSO OPTIMIZES M

FIT ...

184 7040 THE C IN COLUMN 1 OF CARD 10 SHOULD BE REMOVED AND C

INSERTED IN COLUMN 1 OF CARDS 1 AND 11 00000000 0000 o วววว 11 12

13 16 17 18 19 PROGRAM LSO OPTIMIZES M

**PARAMETERS**TO GIVE BESTFIT ...

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absorption acid addition angle appears application approximately B-structure band becomes calculated CD spectra Chem compared complex component concentration conformation constant containing contribution corresponding Cotton effects density dependence derived described determined diffusible discussed electron enzyme equation equilibrium estimate et al example experimental experiments expression factor field function given gives helical helix increased indicated intensity interaction length magnetic measurements mechanism method molecular weight molecule negative observed obtained optical activity osmotic parameters partial positive possible pressure procedure protein protein solution quantum mechanical radius range reference region relative residues respectively rotational strength sample scattering curve shape shown shows side similar solution solvent specific volume spectrum structure studies Table temperature theoretical theory tion transition transition dipole moment usually various vary volume wavelength X-ray zero