Methods in Endothelial Cell Biology

Front Cover
Hellmut G. Augustin
Springer Science & Business Media, Jul 20, 2004 - Medical - 383 pages

Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most important human diseases, including ischemic heart diseases, hypertension, atherosclerosis, tumors, diabetes, arthritis, and inflammation. Further, the increasing importance and recognition of the field of vascular biology in general requires in vitro and in vivo techniques in order to address the complex questions.

Methods in Endothelial Cell Biology is a comprehensive practical "how-to"-guide summarizing the most relevant established techniques as well as a number of new emerging techniques. Easy-to-follow reliable protocols provide a useful lab bench resource for the experienced researcher and newcomer to the field.

 

Contents

Isolation Purification and Culture of Human Micro and Macrovascular Endothelial Cells
3
Isolation of Mouse Cardiac Microvascular Endothelial Cells
15
Isolation Culture and Differentiation of Circulating Endothelial Precursor Cells
23
Embryonic Stem CellDerived Endothelial Cells
33
Detection Isolation and Culture of Lymphatic Endothelial Cells
47
ThreeDimensional Spheroid Culture of Endothelial Cells
55
Immortalization of Endothelial Cells
63
Viral Gene Transfer Into Endothelial Cells
73
Peripheral Hind Limb Ischemia Models
197
In Vivo Matrigel Angiogenesis Assays
207
Analysis of Vascular Permeability in Vivo
223
Validating the Manipulation of Endothelial Cell Signaling in Vivo
233
Chick Chorioallantoic Membrane Assay Growth Factor and Tumorinduced Angiogenesis and Lymphangiogenesis
247
Corneal Angiogenesis Assay
263
Detection of Endothelial Cells
273
Basic Blood Vessel Immunohistochemistry
275

Functional Assays in Vitro
83
Leukocyte Adhesion to Endothelium in Vitro Under Shear Stress
85
Application of Biomechanical Forces on Endothelial Cells
93
Endothelial Cell Permeability Assays in Culture
103
ThreeDimensional in Vitro Angiogenesis Assays
115
Rat Aortic Ring Assay of Angiogenesis
125
Endothelial Cell Chemotaxis and Chemokinesis Assays
145
FACS Analysis of Endothelial Cells
157
Application of the RNA Interference RNAi Technology to Angiogenesis Research
167
Functional Assays in Vivo
177
Murine Bone Marrow Transplantation Models that Enable the Study of EPC Recruitment
179
Balloon Denudation of Blood Vessels
187
DoubleStaining Immunohistochemistry of Blood Vessels
287
In Situ Hybridization Analysis of Vascular Endothelium
301
HighResolution in Situ Confocal Analysis of Endothelial Cells
313
Whole Mount Analysis of the Embryonic Vasculature
325
Methods for Visualizing Intact Blood Vessels Using Immunofluorescent Localization of PECAM CD31 and aSmooth Muscle Actin
337
Intravital Multifluorescence Microscopy
347
Corrosion Cast Analysis of Blood Vessels
357
Appendix
371
Suppliers
373
Endothelial Cell Biology on the Web
377
Subject Index
379
Copyright

Other editions - View all

Common terms and phrases