Physical Principles and Techniques of Protein Chemistry, Part 1Sydney J. Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ... |
From inside the book
Results 1-3 of 79
Page 9
... optical paths ( refractive index X distance ) before reaching the image . If variations in optical path occur , variations in the in- tensity of image points are produced by phase interference between co- incident rays which differ in ...
... optical paths ( refractive index X distance ) before reaching the image . If variations in optical path occur , variations in the in- tensity of image points are produced by phase interference between co- incident rays which differ in ...
Page 336
... optical anisotropy factor light intensity Boltzmann constant Kerr constant , An / nE2 optical path length torque refractive index principal refractive indices parallel and perpendicular to the electric field , respectively ...
... optical anisotropy factor light intensity Boltzmann constant Kerr constant , An / nE2 optical path length torque refractive index principal refractive indices parallel and perpendicular to the electric field , respectively ...
Page 339
... optical anisotropy factor from the field strength dependence of the birefringence in the case of axially symmetric rigid macromolecules . This method has been applied to tobacco mosaic virus , synthetic polypeptides , and collagen ...
... optical anisotropy factor from the field strength dependence of the birefringence in the case of axially symmetric rigid macromolecules . This method has been applied to tobacco mosaic virus , synthetic polypeptides , and collagen ...
Contents
Electron Microscopy of Globular Proteins | 2 |
Ultraviolet Absorption | 3 |
The Enhancement of Contrast | 21 |
Copyright | |
45 other sections not shown
Other editions - View all
Common terms and phrases
absorption absorption spectrum applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient components concentration curve denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental factor film fluorescence fraction frequency gel filtration gradient groups intensity interactions ionic strength ions lens light linear macromolecules measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone