Molecular Cloning: A Laboratory Manual, Book 3

acetate activity added Adjust aliquots allow amount antibody antigen Appendix appropriate approximately aqueous assay bacterial bacteriophage base binding buffer carried cells centrifugation Chapter chromatography Cl pH cloned coli column complete concentration containing culture described desired detected dilution Dissolve EDTA efficiency electrophoresis enzyme et al ethanol expression extract Figure filter final foreign fragment fusion gene growth hybridization Incubate levels lines mammalian cells medium method mg/ml minutes mixture NaCl nucleic acid obtained phase phosphate Place plasmid plate polymerase precipitation prepared presence procedure production promoter protein radioactive radiolabeled reaction remove restriction room temperature sample screen selection sequences sodium solution specific step sterile stored strain TABLE Telephone tion transcription transfected transfer transformation Tris ug/ml usually vectors volume wash western blotting ОН