Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 12
Page A-2
... Adjust the pH to 7.0 with 5 N NaOH ( ~ 0.2 ml ) . Adjust the volume of the solution to 1 liter with deionized H2O . Sterilize by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . Just before use , add 5 ml of a sterile ...
... Adjust the pH to 7.0 with 5 N NaOH ( ~ 0.2 ml ) . Adjust the volume of the solution to 1 liter with deionized H2O . Sterilize by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . Just before use , add 5 ml of a sterile ...
Page B-11
... Adjust the volume of the solution to 10 ml with distilled H2O and sterilize by filtration through a 0.22- micron disposable filter . Dispense the solution into 1 - ml aliquots and store them at -20 ° C . TABLE B.7 ( continued ) Solution ...
... Adjust the volume of the solution to 10 ml with distilled H2O and sterilize by filtration through a 0.22- micron disposable filter . Dispense the solution into 1 - ml aliquots and store them at -20 ° C . TABLE B.7 ( continued ) Solution ...
Page B-12
... Adjust the volume to 1 liter with H2O . Sterilize by filtration . Dissolve 203.3 g of MgCl ̧ · 6H2O in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Dispense into aliquots and sterilize by auto- claving . Usually obtained as a ...
... Adjust the volume to 1 liter with H2O . Sterilize by filtration . Dissolve 203.3 g of MgCl ̧ · 6H2O in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Dispense into aliquots and sterilize by auto- claving . Usually obtained as a ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml