Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1676
... Biol . 7 : 1568 . Kaufman , R.J. and P.A. Sharp . 1982a . Amplification and expression of se- quences cotransfected with a modular dihydrofolate reductase complemen- tary DNA gene . J. Mol . Biol . 159 : 601 . 1982b . Construction of a ...
... Biol . 7 : 1568 . Kaufman , R.J. and P.A. Sharp . 1982a . Amplification and expression of se- quences cotransfected with a modular dihydrofolate reductase complemen- tary DNA gene . J. Mol . Biol . 159 : 601 . 1982b . Construction of a ...
Page 1678
... Biol . 2 : 161 . - . 1983. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells . Mol . Cell . Biol . 3 : 280 . 1985. Bacteriophage lambda vec- tor for transducing a cDNA clone li- brary into mammalian cells ...
... Biol . 2 : 161 . - . 1983. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells . Mol . Cell . Biol . 3 : 280 . 1985. Bacteriophage lambda vec- tor for transducing a cDNA clone li- brary into mammalian cells ...
Page 1680
... Biol . 6 : 1074 . Simonsen , C.C. and A.D. Levinson . 1983 . Isolation and expression of an altered mouse dihydrofolate reductase cDNA . Proc . Natl . Acad . Sci . 80 : 2495 . Simonsen , C.C. , H.M. Shepard , P.W. Gray , D.W. Leung , D ...
... Biol . 6 : 1074 . Simonsen , C.C. and A.D. Levinson . 1983 . Isolation and expression of an altered mouse dihydrofolate reductase cDNA . Proc . Natl . Acad . Sci . 80 : 2495 . Simonsen , C.C. , H.M. Shepard , P.W. Gray , D.W. Leung , D ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml