Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 172
... chapter , we describe methods and plasmid vectors for producing fusion proteins and intact native proteins in bacteria . Fusion proteins can be made in large amounts , are easy to purify , and can be used to elicit an antibody response ...
... chapter , we describe methods and plasmid vectors for producing fusion proteins and intact native proteins in bacteria . Fusion proteins can be made in large amounts , are easy to purify , and can be used to elicit an antibody response ...
Page 17-2
... chapter , we describe methods and plasmid vectors for producing fusion proteins and intact native proteins in bacteria . Fusion proteins can be made in large amounts , are easy to purify , and can be used to elicit an antibody response ...
... chapter , we describe methods and plasmid vectors for producing fusion proteins and intact native proteins in bacteria . Fusion proteins can be made in large amounts , are easy to purify , and can be used to elicit an antibody response ...
Page 17-25
... Chapter 1 ) . For pKK240-11 , use strain RB791 ; for pAS1 , use M5219 . 4. Screen transformants for colonies that contain the cloned gene by plasmid minipreps and restriction enzyme analysis . Sequence the junction be- tween the ATG and ...
... Chapter 1 ) . For pKK240-11 , use strain RB791 ; for pAS1 , use M5219 . 4. Screen transformants for colonies that contain the cloned gene by plasmid minipreps and restriction enzyme analysis . Sequence the junction be- tween the ATG and ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml