Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 55
Page 1578
... DNA Mapping 5.26 DIGESTING DNA WITH RESTRICTION ENZYMES 5.28 Setting Up Digestions with Restriction Enzymes 5.31 Other Enzymes Used in Molecular Cloning 5.33 DNA POLYMERASES 5.35 DNA Polymerase I ( Holoenzyme ) 5.36 Large Fragment of DNA ...
... DNA Mapping 5.26 DIGESTING DNA WITH RESTRICTION ENZYMES 5.28 Setting Up Digestions with Restriction Enzymes 5.31 Other Enzymes Used in Molecular Cloning 5.33 DNA POLYMERASES 5.35 DNA Polymerase I ( Holoenzyme ) 5.36 Large Fragment of DNA ...
Page F-4
... DNA polymerase I and bacteriophage T4 DNA polymerase carry a 3 ' → 5 ' exonuclease activity that can be used to remove protruding nucleotides from the 3 ' termini of DNA . Both enzymes lack a 5 ' - 3 ' exonuclease activity and carry a ...
... DNA polymerase I and bacteriophage T4 DNA polymerase carry a 3 ' → 5 ' exonuclease activity that can be used to remove protruding nucleotides from the 3 ' termini of DNA . Both enzymes lack a 5 ' - 3 ' exonuclease activity and carry a ...
Page 1-43
... DNA polymerase I ; T4 bacteriophage , DNA polymerase Subtracted probes . See Probes Sucrose density gradients containing methylmercuric hydroxide , for RNA , 7.35-7.36 for purification of bacteriophage › arms , 2.85-2.87 Sulfuric acid ...
... DNA polymerase I ; T4 bacteriophage , DNA polymerase Subtracted probes . See Probes Sucrose density gradients containing methylmercuric hydroxide , for RNA , 7.35-7.36 for purification of bacteriophage › arms , 2.85-2.87 Sulfuric acid ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml