Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 35
Page 1633
... Dissolve 1.6 g of NaCl , 0.074 g of KCl , 0.027 g of Na2HPO4 2H2O , 0.2 g of dextrose , and 1 g of HEPES in a total volume of 90 ml of distilled H2O . Adjust the pH to 7.05 with 0.5 N NaOH , and then adjust the volume to 100 ml with ...
... Dissolve 1.6 g of NaCl , 0.074 g of KCl , 0.027 g of Na2HPO4 2H2O , 0.2 g of dextrose , and 1 g of HEPES in a total volume of 90 ml of distilled H2O . Adjust the pH to 7.05 with 0.5 N NaOH , and then adjust the volume to 100 ml with ...
Page B-9
... Dissolve 29 g of acrylamide and 1 g of N , N'- methylenebisacrylamide in a total volume of 60 ml of H2O . Heat the solution to 37 ° C to dissolve the chemicals . Adjust the volume to 100 ml with H2O . Sterilize the solution by ...
... Dissolve 29 g of acrylamide and 1 g of N , N'- methylenebisacrylamide in a total volume of 60 ml of H2O . Heat the solution to 37 ° C to dissolve the chemicals . Adjust the volume to 100 ml with H2O . Sterilize the solution by ...
Page B-12
... Dissolve 8 g of NaCl , 0.2 g of KCl , 1.44 g of Na2HPO4 , and 0.24 g of KH2PO , in 800 ml of distilled H2O . Adjust ... Dissolve 214.46 g of magnesium acetate 4H2O in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Sterilize by ...
... Dissolve 8 g of NaCl , 0.2 g of KCl , 1.44 g of Na2HPO4 , and 0.24 g of KH2PO , in 800 ml of distilled H2O . Adjust ... Dissolve 214.46 g of magnesium acetate 4H2O in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Sterilize by ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml